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PEER REVIEW 4:Experimental Assessment of Moringa oleifera Leaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential Using In Vitro and In Vivo Assays

For hundreds of years, traditional healers have prescribed different parts of M. oleifera for treatment of skin diseases, respiratory illnesses, ear and dental infections, hypertension, diabetes, cancer treatment, and water purification, and they have promoted its use as a nutrient dense food source [6, 10, 12]. Herein, we report a comparative in vitro and in vivo analysis of the effect of aqueous and ethanolic extracts of Moringa leaf and fruit on the markers of oxidative stress. Biomarkers of oxidative stress reflect environmental pro-oxidant and antioxidant ratio and also serve as a surrogate measure of a disease process. The protective effects of aqueous and ethanolic extract of Moringa leaf and fruit on erythrocyte GSH and MDA concentration may be attributed to the presence of phytoconstituents (polyphenols, tannins, anthocyanin, glycosides, thiocarbamates) that scavenge free radicals, activate the antioxidant enzymes, and inhibit oxidases [55, 56]. In present study, we studied both concentration- and dose-dependent analysis by estimating GSH and MDA concentration in vitro as well as in vivo.

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PEER REVIEW 4:Experimental Assessment of Moringa oleifera Leaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential Using In Vitro and In Vivo Assays

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